Research

Monitoring photosynthesis on canopy level using proximal remote sensing of chlorophyll fluorescene yield. By Daniel Bånkestad from Heliospectra, Linnéa Ahlman & Torsten Wik from Chalmers University of Technology and Karl-Johan Bergstrand & Jean W. H. Yong from Swedish University of Agricultural Sciences. 

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Introduction

Chlorophyll fluorescence is interesting and useful for phenotyping applications as it is rich in biological information and measurable remotely and non-destructively. Several tools measuring and analyzing this signal exist, whereof PAM fluorometry is most widely used. Here we explore a different approach, measuring fluorescence yield (F yield; also termed fluorescence gain) at the canopy level, actively induced by a weak excitation light. The study focuses on the interplay between F yield on canopy level and leaf photosynthesis using light response curve data. One of the research questions was, does the peak in fluorescence yield correlate with saturation of photosynthesis?

Conclusions

  • The study highlights a concave fluorescence-light link, peaking when photosynthesis saturates, especially in sunflowers.
  • This method swiftly assesses plant stress and light tolerance in the field, without dark adaptation or saturating light, using only weak excitation light.
  • Its high-throughput potential benefits large-scale applications, enhancing our grasp of plant stress and tolerance.
  • Compared to PAM fluorometry, it’s a more efficient and field-friendly approach.

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